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Products:Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Overview
Product name
Anti-Laminin alpha 4 antibody
See all Laminin alpha 4 products (2) ...
Description
Mouse polyclonal to Laminin alpha 4
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-121 of Human Laminin alpha 4
Positive control
293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Other ECM Proteins
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
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Western blot - Laminin alpha 4 antibody (ab67072)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 203 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificity
In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis.
Sequence similarities
Contains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
Target information above from: UniProt accessionQ16363
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686D23145 antibody
- LAMA3 antibody
- LAMA4 1 antibody
see all
Database links
- Entrez Gene: 3910 Human
- Omim: 600133 Human
- SwissProt: Q16363 Human
- Unigene: 654572 Human
Anti-Laminin alpha 4 antibody images:
Western blot - Laminin alpha 4 antibody (ab67072)
All lanes : Anti-Laminin alpha 4 antibody (ab67072) at 1/500 dilution
Lane 1 : LAMA4 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
Goat Anti Mouse IgG (H&L) HRP Conjugate at 1/2500 dilution
Predicted band size : 203 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
References for Anti-Laminin alpha 4 antibody (ab67072)
ab67072 has not yet been referenced specifically in any publications.
Publishing research using ab67072? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"