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Western blot - LAMA4 antibody (ab69634)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMA4 antibody (ab69634)
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Immunocytochemistry/ Immunofluorescence - LAMA4 antibody (ab69634)
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Product Name
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LAMA4 antibody
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See all LAMA4 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to LAMA4
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Immunogen
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Synthesized peptide derived from the internal region of human LAMA4.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 95, 150 and >170 kDa (predicted molecular weight: 203 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH/3T3 cells, human heart tissue and extracts from COLO and 293 cells.
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Cellular localization
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Secreted, extracellular space, extracellular matrix, basement membrane.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Other ECM Proteins
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
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Relevance
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Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination, laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their a chain, are expressed in a cell and tissue specific manner, and are differentially recognized by integrins.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LAMA4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LAMA4 antibody images, references, products related to ab69634 and other tools.
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LAMA4 antibody images:
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 Western blot - LAMA4 antibody (ab69634)
All lanes : LAMA4 antibody (ab69634) at 1/500 dilution
Lane 1 : extracts from COLO 205 cells
Lane 2 : extracts from 293 cells
Lane 3 : extracts from COLO 205 cells plus with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 203 kDa
Observed band size : >170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa,95 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMA4 antibody (ab69634)
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab69634 at 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
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Immunocytochemistry/ Immunofluorescence - LAMA4 antibody (ab69634)
Immunofluorescence analysis of NIH/3T3 cells using ab69634 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
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Search PubMed (MEDLINE) for references to LAMA4
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LAMA4 antibody - more information
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Customer reviews (feedback) regarding LAMA4 antibody |
Customer FAQs regarding LAMA4 antibody |
Protocols for LAMA4 antibody |
Price and availability of products related to LAMA4 antibody |
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LAMA4 antibody - related products:
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LAMA4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about LAMA4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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