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Products:Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Overview
Product name
Anti-Laminin alpha 4 antibody
See all Laminin alpha 4 products (2) ...
Description
Rabbit polyclonal to Laminin alpha 4
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthesized peptide derived from the internal region of human Laminin alpha 4.
Positive control
NIH/3T3 cells, human heart tissue and extracts from COLO and 293 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Other ECM Proteins
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 95, 150 , >170 kDa (predicted molecular weight: 203 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.(Pepsin digestion was used successfully in PMID: 19969511.)
ICC/IF: 1/500 - 1/1000.
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificity
In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis.
Sequence similarities
Contains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domain G is globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
Target information above from: UniProt accessionQ16363
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686D23145 antibody
- LAMA3 antibody
- LAMA4 1 antibody
see all
Database links
- Entrez Gene: 3910 Human
- Entrez Gene: 16775 Mouse
- Omim: 600133 Human
- SwissProt: Q16363 Human
- SwissProt: P97927 Mouse
- Unigene: 654572 Human
- Unigene: 258065 Mouse
Anti-Laminin alpha 4 antibody images:
Western blot - Laminin alpha 4 antibody (ab69634)
All lanes : Anti-Laminin alpha 4 antibody (ab69634) at 1/500 dilution
Lane 1 : extracts from COLO 205 cells
Lane 2 : extracts from 293 cells
Lane 3 : extracts from COLO 205 cells plus with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 203 kDa
Observed band size : >170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 150 kDa,95 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Laminin alpha 4 antibody (ab69634)
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab69634 at 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Laminin alpha 4 antibody (ab69634)
Immunofluorescence analysis of NIH/3T3 cells using ab69634 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
References for Anti-Laminin alpha 4 antibody (ab69634)
This product has been referenced in:
- Snipstad Ket al. New specific molecular targets for radio-chemotherapy of rectal cancer. Mol Oncol 4:52-64 (2010). IHC-P; Human.Read more (PubMed: 19969511) »
See 1 publication for this product
Publishing research using ab69634? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"