LAMP1 antibody - Drosophila Lysosome Marker (ab32343)

Product Name 

LAMP1 antibody - Drosophila Lysosome Marker

Product type 

Primary antibodies

Description 

Rabbit polyclonal to LAMP1 - Drosophila Lysosome Marker

Immunogen 

Synthetic peptide derived from within residues 200 - 300 of Fruit fly (Drosophila melanogaster) LAMP1.

(Peptide available as ab32342.)

Reacts with 
(species key)

Dm

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/500. Detects a band of approximately 130 kDa (predicted molecular weight: 35 kDa for the core protein but an expected size of 120-140 kDa due to glycosylation).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Schneider L2 (Drosophila melanogaster) Whole cell lysate;

Cellular localization 

Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Research areas 

Cancer >> Cancer Metabolism >> Autophagy
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.300 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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