Overview

  • Product nameAnti-LAMP1 antibody [EPR4204]
    See all LAMP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4204] to LAMP1
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human LAMP1.

  • Positive control
    • HeLa, JAR, Jurkat, and A431 cell lysates; Human kidney tissue

Applications

Our Abpromise guarantee covers the use of ab108597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • Sequence similaritiesBelongs to the LAMP family.
    • Post-translational
      modifications
      O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    Anti-LAMP1 antibody [EPR4204] images

    • All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 45 kDa
    • Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using ab108597 at a dilution of 1/100.

    References for Anti-LAMP1 antibody [EPR4204] (ab108597)

    This product has been referenced in:
    • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . Read more (PubMed: 23856606) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Human Cell lysate - whole cell (Cortical tissue)
    Specification Cortical tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Martin Broadstock

    Verified customer

    Submitted Jun 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (BA9 cortical sections)
    Specification BA9 cortical sections
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization Yes - 0.05% Tween-20
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Dr. Martin Broadstock

    Verified customer

    Submitted May 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"