Overview

  • Product nameAnti-LAMP1 antibody [EPR4204]
    See all LAMP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4204] to LAMP1
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human LAMP1.

  • Positive control
    • HeLa, JAR, Jurkat, and A431 cell lysates; Human kidney tissue
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab108597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionPresents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
    • Sequence similaritiesBelongs to the LAMP family.
    • Post-translational
      modifications
      O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
    • Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD107 antigen like family member A antibody
      • CD107 antigen-like family member A antibody
      • CD107a antibody
      • CD107a antigen antibody
      • LAMP 1 antibody
      • LAMP-1 antibody
      • LAMP1 antibody
      • LAMP1_HUMAN antibody
      • LAMPA antibody
      • LGP120 antibody
      • lgpA antibody
      • Lysosomal membrane glycoprotein 120KD antibody
      • Lysosomal Associated Membrane Protein 1 antibody
      • Lysosome associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane glycoprotein 1 antibody
      • Lysosome-associated membrane protein 1 antibody
      • OTTHUMP00000040663 antibody
      see all

    Anti-LAMP1 antibody [EPR4204] images

    • All lanes : Anti-LAMP1 antibody [EPR4204] (ab108597) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 45 kDa
    • Immunohistochemical analysis of LAMP1 in paraffin embedded Human kidney tissue, using ab108597 at a dilution of 1/100.

    References for Anti-LAMP1 antibody [EPR4204] (ab108597)

    This product has been referenced in:
    • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . Read more (PubMed: 23856606) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Human Cell lysate - whole cell (Cortical tissue)
    Specification Cortical tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Martin Broadstock

    Verified customer

    Submitted Jun 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (BA9 cortical sections)
    Specification BA9 cortical sections
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization Yes - 0.05% Tween-20
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Dr. Martin Broadstock

    Verified customer

    Submitted May 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"