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Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)
![Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)](/ps/datasheet/images/ab25630_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP1 antibody [H4A3] - Lysosome Marker(ab25630)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP1 antibody [H4A3] - Lysosome Marker(ab25630)](\ps\datasheet\images\25\ab25630\LAMP1-Primary-antibodies-ab25630-4.jpg)
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Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)
![Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)](/ps/datasheet/images/25/ab25630/LAMP1-Primary-antibodies-ab25630-5.jpg)
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Product Name
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LAMP1 antibody [H4A3] - Lysosome Marker
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See all LAMP1 antibodies (19)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [H4A3] to LAMP1 - Lysosome Marker
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Immunogen
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The details of the immunogen for this antibody are not available.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 1µg for 106 cells.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
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Research areas
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Cancer >> Cancer Metabolism >> Autophagy
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Relevance
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Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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H4A3
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: None
Constituents: 100mM Borate. pH 8.0
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LAMP1 antibody [H4A3] - Lysosome Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LAMP1 antibody [H4A3] - Lysosome Marker images, references, products related to ab25630 and other tools.
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LAMP1 antibody [H4A3] - Lysosome Marker images:
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 Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)
ab25630 at 1/100 staining human Primary Gingival epithelial cells by ICC/IF. The cells were ixed with 4% paraformaldehyde, permeabilized with 0.5% saponin and then blocked overnight with 10% goat serum, 5% BSA. The cells were incubated with the antibody for 1 hour and then a FITC conjugated goat polyclonal antibody was used as the secondary. The cells were counterstaind with DAPI for the nucleus and Cell Tracker Blue for the cytoplasm. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP1 antibody [H4A3] - Lysosome Marker(ab25630)
Ab25630 staining Human normal placenta. Staining is localized to the cell membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)
ab25630 staining LAMP1 in human HaCaT keratinocytes by Immunocytochemistry/ immunofluorescence. Cells were fixed with acetone, permeabilized with ice cold acetone and blocking with 4% PBS, 0.4% BSA and goat serum was done. Samples were incubated with primary antibody (1/100: in 10% blocking solution in PBS) for 1 hour at 230C. An Alexa Fluor ® 680-conjugated goat polyclonal to mouse IgG, diluted 1/200 was used as secondary antibody. This image is a courtesy of Anonimous Abreview.
See Abreview
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References for LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)
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This product has been used in: (two most recent references)
Heidtman M et al. Large-scale identification of Legionella pneumophila Dot/Icm substrates that modulate host cell vesicle trafficking pathways. Cell Microbiol 11:230-48 (2009). ICC/IF; Human. PubMed: 19016775
Thurston TL et al. The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria. Nat Immunol 10:1215-21 (2009). ICC/IF; Human. PubMed: 19820708
See all 6 publication references for this product.
If you publish research using ab25630 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to LAMP1
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LAMP1 antibody [H4A3] - Lysosome Marker - more information
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LAMP1 antibody [H4A3] - Lysosome Marker - related products:
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LAMP1 antibody [H4A3] - Lysosome Marker - other tools:
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Contact Abcam with a Technical Enquiry about LAMP1 antibody [H4A3] - Lysosome Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)](/index.html?datasheet=25630#ab25630_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP1 antibody [H4A3] - Lysosome Marker(ab25630)](/index.html?datasheet=25630#LAMP1-Primary-antibodies-ab25630-4.jpg)
![Immunocytochemistry/ Immunofluorescence - LAMP1 antibody [H4A3] - Lysosome Marker (ab25630)](/index.html?datasheet=25630#LAMP1-Primary-antibodies-ab25630-5.jpg)
