LAMP1 antibody [LY1C6] - Lysosome Marker (ab13523)

Product Name 

LAMP1 antibody [LY1C6] - Lysosome Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [LY1C6] to LAMP1 - Lysosome Marker

Immunogen 

Rat liver lysosomal membrane preparations

Reacts with 
(species key)

Ms, Rat, Hm

Tested applications 
(see key)

ICC, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/100 - 1/750. Use a PFA based fixative (see enquiries).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Highly purified rat lysosomal membrane preparations.

Cellular localization 

Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Research areas 

Cancer >> Cancer Metabolism >> Autophagy
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Relevance 

Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

LY1C6

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

For maximum product recovery centrifuge the product vial before removing cap.

References for LAMP1 antibody [LY1C6] - Lysosome Marker (ab13523)

  This product has been used in: (two most recent references)

Pfender NA et al. Route of Uptake of Palmitoylated Encephalitogenic Peptides of Myelin Proteolipid Protein by Antigen-Presenting Cells: Importance of the Type of Bond between Lipid Chain and Peptide and Relevance to Autoimmunity. J Immunol 180:1398-404 (2008). ICC/IF; Mouse. PubMed: 18209034

Lanaspa MA et al. Hypertonic stress increases claudin-4 expression and tight junction integrity in association with MUPP1 in IMCD3 cells. Proc Natl Acad Sci U S A 105:15797-802 (2008). ICC/IF; Mouse. PubMed: 18840681

See all 9 publication references for this product.


If you publish research using ab13523 please let us know so that we can cite the reference on this datasheet.

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