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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab62562? Please let us know so that we can cite the reference in this datasheet
ab62562 has been referenced in 2 publications.
- Abramowski D et al. Transgenic expression of intraneuronal Aß42 but not Aß40 leads to cellular Aß lesions, degeneration, and functional impairment without typical Alzheimer's disease pathology. J Neurosci 32:1273-83 (2012). ICC/IF; Mouse. PubMed: 22279212
- Jabbour M et al. Discrete domains of MARCH1 mediate its localization, functional interactions, and posttranscriptional control of expression. J Immunol 183:6500-12 (2009). ICC/IF; Mouse. PubMed: 19880452
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - LAMP1 antibody (ab62562)
Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 45 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Despite its predicted size, LAMP1 migrates at 110kDa in SDS-PAGE.
There are multiple additional bands. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP1 antibody(ab62562)
Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab62562)
ab62562, at 20µg/ml, staining LAMP1 in human colon tissue by immunofluorescence.
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