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Does this antibody crossreact with bovine? Can you maybe provide a sequence alignment of the immunogen with the bovine protein or the score of homology? Thank you very much! |
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ANSWER: |
The immunogen sequence is unfortunately proprietary information. The laboratory however made an alignement to predict crossreactivity. There is 66.7% homology with bovine [* ** * ** **** ]. The accession number used for the alignment was NP_001068592.1. It is therefore very unlikely that this antibody will react with the bovine protein. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 45 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Despite its predicted size, LAMP1 migrates at 110kDa in SDS-PAGE.
There are multiple additional bands. We are unsure as to the identity of these extra bands.
Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab62562, at 20µg/ml, staining LAMP1 in human colon tissue by immunofluorescence.
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