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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-LAMP1 antibody
See all LAMP1 products (21) ...
Description
Rabbit polyclonal to LAMP1
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A 15 amino acid peptide from near the centre of human LAMP1.
Positive control
EL4 cell lysate. Human colon tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Autophagy
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
ICC/IF: Use a concentration of 20 µg/ml
Target
Function
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Sequence similarities
Belongs to the LAMP family.
Post-translational
modifications
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Target information above from: UniProt accessionP11279
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD107 antigen like family member A antibody
- CD107 antigen-like family member A antibody
- CD107a antibody
see all
Database links
- Entrez Gene: 3916 Human
- Entrez Gene: 16783 Mouse
- Entrez Gene: 25328 Rat
- Omim: 153330 Human
- SwissProt: P11279 Human
- SwissProt: P11438 Mouse
- SwissProt: P14562 Rat
- Unigene: 494419 Human
see all
Anti-LAMP1 antibody images:
Western blot - LAMP1 antibody (ab62562)
Lane 1 : Anti-LAMP1 antibody (ab62562) at 1 µg/ml
Lane 2 : Anti-LAMP1 antibody (ab62562) at 2 µg/ml
Lane 1 : EL4 cell lysate
Lane 2 : EL4 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 45 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Despite its predicted size, LAMP1 migrates at 110kDa in SDS-PAGE.
There are multiple additional bands. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP1 antibody(ab62562)
Ab62562 staining human normal placenta. Staining is localised to cytoplasmic and nucleus compartiments.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - LAMP1 antibody (ab62562)
ab62562, at 20µg/ml, staining LAMP1 in human colon tissue by immunofluorescence.
References for Anti-LAMP1 antibody (ab62562)
This product has been referenced in:
- Jabbour Met al. Discrete domains of MARCH1 mediate its localization, functional interactions, and posttranscriptional control of expression. J Immunol 183:6500-12 (2009). ICC/IF; Mouse.Read more (PubMed: 19880452) »
See 1 publication for this product
Publishing research using ab62562? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"