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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Anti-LAMP2 antibody [ABL-93] - Lysosome Marker (ab25339)
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- Secondary antibodies
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab25339 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Frozen sections) - LAMP2 antibody [ABL-93] - Lysosome Marker (ab25339)](/ps/datasheet/Images/25/ab25339/ab25339_1.jpg)
Immunohistochemistry (Frozen sections) - LAMP2 antibody [ABL-93] - Lysosome Marker (ab25339)
ab25339 staining LAMP2 from mouse liver cells by Immunohistochemistry (Frozen sections). The fresh tissue was cut into small pieces, put into OCT, and frozen in liquid nitrogen. 5µm sections were prepared using a cyrostat. The tissue section was fixed in 5% paraformaldehyde and blocked in 5% serum with 0.05% saponin to increase the permeability. The sample was incubated with ab25339 (1/100 dilution) overnight, and incubated with secondary antibody (Cy2 conjugated donkey anti-rat IgG) for 2 hours. After staining with DAPI (1/1000 dilution) for 2 minutess, the section was mounted.
This image is courtesy of an anonymous Abreview
![Flow Cytometry-LAMP2 antibody [ABL-93] - Lysosome Marker(ab25339)](/ps/datasheet/images/25/ab25339/LAMP2-Primary-antibodies-ab25339-1.jpg)
Flow Cytometry-LAMP2 antibody [ABL-93] - Lysosome Marker(ab25339)
Overlay histogram showing RAW 264.7 cells stained with ab25339 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25339, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (
![LAMP2 antibody [ABL-93] - Lysosome Marker for IHC-Fr in Mouse (25339)](/ps/Reviews/Images/ab5243_17378.jpg)
LAMP2 antibody [ABL-93] - Lysosome Marker for IHC-Fr in Mouse (25339)
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