Anti-LAMP2 antibody [ABL-93] - Lysosome Marker (ab25339)

Immunohistochemistry (Frozen sections) - LAMP2 antibody [ABL-93] - Lysosome Marker (ab25339)

ab25339 staining LAMP2 from mouse liver cells by Immunohistochemistry (Frozen sections). The fresh tissue was cut into small pieces, put into OCT, and frozen in liquid nitrogen. 5µm sections were prepared using a cyrostat. The tissue section was fixed in 5% paraformaldehyde and blocked in 5% serum with 0.05% saponin to increase the permeability. The sample was incubated with ab25339 (1/100 dilution) overnight, and incubated with secondary antibody (Cy2 conjugated donkey anti-rat IgG) for 2 hours. After staining with DAPI (1/1000 dilution) for 2 minutess, the section was mounted.

This image is courtesy of an anonymous Abreview

Flow Cytometry-LAMP2 antibody [ABL-93] - Lysosome Marker(ab25339)

Overlay histogram showing RAW 264.7 cells stained with ab25339 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25339, 1µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.