Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
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ab13524 has been referenced in 9 publications.
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Mouse astrocytes stained with Anti-LAMP2 ab13524. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.
All lanes : Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524) at 1/300 dilution
Lane 1 : Human U2OS - whole cell lysate
Lane 2 : Human 293T - whole cell lysate
Lane 3 : Monkey Cos-7 - whole cell lysate
Lane 4 : Hamster CHO - whole cell lysate
Lane 5 : Rat PC12 - whole cell lysate
Lane 6 : Mouse 3T3 - whole cell lysate
Secondary
HRP conjugated rabbit anti-rat antibody
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an anonymous Abreview
Overlay histogram showing THP1 cells stained with ab13524 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13524, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (
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