Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

Immunocytochemistry - LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

Mouse astrocytes stained with Anti-LAMP2 ab13524. Cells were fixed using paraformaldehyde-lysine-periodate (including 4% paraformaldehyde) and permeabilized using 0.01% saponin.

Western blot - LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

All lanes : Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524) at 1/300 dilution

Lane 1 : Human U2OS - whole cell lysate
Lane 2 : Human 293T - whole cell lysate
Lane 3 : Monkey Cos-7 - whole cell lysate
Lane 4 : Hamster CHO - whole cell lysate
Lane 5 : Rat PC12 - whole cell lysate
Lane 6 : Mouse 3T3 - whole cell lysate

Secondary
HRP conjugated rabbit anti-rat antibody

Observed band size : 105 kDa (why is the actual band size different from the predicted?)

This image is courtesy of an anonymous Abreview

Flow Cytometry-Anti-LAMP2 antibody [GL2A7] - Lysosome Marker(ab13524)

Overlay histogram showing THP1 cells stained with ab13524 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13524, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.