LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

Product Name 

LAMP2 antibody [GL2A7] - Lysosome Marker

Product type 

Primary antibodies

Description 

Rat monoclonal [GL2A7] to LAMP2 - Lysosome Marker

Immunogen 

Purified preparation of mouse liver lysosomal membranes.

Reacts with 
(species key)

Hu, Ms, Rb

Tested applications 
(see key)

ICC, IHC-FoFr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: 1/100. Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.
IHC-FoFr: 1/100 (PubMed:17425404).
IP: Use at an assay dependent dilution (PMID 2142158).
WB: Use at an assay dependent dilution (see Abreview).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

mouse astrocytes

Cellular localization 

Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Myeloid Progenitor
Cardiovascular >> Heart >> Autophagy >> Autophagosome
Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome

Relevance 

Lysosomes are membrane bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to post-translational modification, Lysosome Associated Membrane Protein 2 (LAMP2) is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino terminal intra lysosomal domain, a hydrophobic transmembrane domain, and a short carboxyl terminal cytoplasmic tail. Studies suggest that LAMP2 may be involved in leucocyte adhesion.
LAMP2 has recently been shown to be important in autophagy - the process of bulk degradation of cellular proteins. Human LAMP-2 deficiency causes Danon’s disease which is associated with the accumulation of autophagic
material in striated myocytes.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

GL2A7

Isotype 

IgG

Purity 

Ascites

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

See below for LAMP2 antibody [GL2A7] - Lysosome Marker images, references, products related to ab13524 and other tools.

LAMP2 antibody [GL2A7] - Lysosome Marker images:

References for LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

  This product has been used in: (two most recent references)

Demonbreun AR et al. Myoferlin is required for insulin-like growth factor response and muscle growth. FASEB J : (2009). PubMed: 20008164

Takahashi K et al. TREM2-transduced myeloid precursors mediate nervous tissue debris clearance and facilitate recovery in an animal model of multiple sclerosis. PLoS Med 4:e124 (2007). PubMed: 17425404

See all 4 publication references for this product.


If you publish research using ab13524 please let us know so that we can cite the reference on this datasheet.

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