Anti-LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)

ab25630: WB with rat liver
sees band at 25 kDa
transfer is OK, detects other proteins in the blot
Ab: 1/1000 1-2 h RT
block: overnight 4 degree
secondary: OK
antibody from other company to same protein gives same results
suggestions: WB conditions might not yet be optimal, try blocking for only 1-2 h RT, antibody incubation: overnight 4 degree
ab25631: rat is unsuitable
ab2773: WB with rat liver, sees band at 25 kDa
suggestions: WB conditions might not yet be optimal, try blocking for only 1-2 h RT, antibody incubation: overnight 4 degree

ANSWER:

Thank you for contacting us.
I am sorry to hear about the problems you have encountered.

The bands you are seeing at 25 kDa could be the light chain of endogenous IgG.

As we discussed over the phone, please try the following suggestions as it seems the WB conditions for these 2 antibodies (ab25630 and ab2773) are not yet optimal:

1) try blocking for only 1-2 h RT
2) try the primary antibody incubation for overnight 4 degree

As for ab25631, this has been shown to not react with rat samples and would probably not give satisfactory results.However, if you have access to a human sample, you could try the antibody on that.

Please let me know if the tips were helping to improve your results.

I hope this information is helpful to you, and I look forward to hear back.

Hi, The power cut was not long than 24 hours and we cannot assure if the products were or not at room temperature when the electricity switched again (they were kept in the fridge but it was not working as a consequence of the power cut). We need your evaluation about the state of the products, because if they are damaged we will claim it cost to the insurance company and we will process with its reposition. Thanks in advance for your help and collaboration Kind regards,    

ANSWER:

Thank you for contacting us.

My colleague "Ariana" has already sent her reply so I am closing this case.

The antibodies are fine for 1 week at room temperature however in your case if the products fail to perform I would suggest getting refund from insurance company. You can get information of product price from our website at  http://www.abcam.com/

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.    

Dear Sir/Madame, Recently IRB Barcelona suffered a power cut and it caused that refrigerators and freezers located in our plant were out of operating. In order to proceed with an official claim for the possible costs that could arise from damaged products as a result of their inadequate storage, we will appreciate your evaluation about the items or products that should be rejected and destroyed because their proper running could not being guaranteed. Please find below the items/products affected Supplier Ref. ITEM Description Abcam ab25631 Ms mAb to LAMP2[H4B4] Abcam ab47517 mtTFA antibody (ab47517 Abcam ab56889 Anticòs monoclonal Mfn2     Thanks in advance for your help and cooperation.   Kind regards.      

ANSWER:

Thank you for contacting us.

In order to help you with your question could you provide the following

- Order number of purchase

- for how long the power cut happened?

-Were these products at room temperature when the electricity switched on again?

What do you really want us to do? Do you need quote for these products please explain?

I look forward to hearing from you soon.

Does antibody H4B4 recognize LAMP-2B?

ANSWER:

Thank you for contacting us.

In humans, the three splice variants (LAMP-2A, LAMP-2B and LAMP-2C) all have identical extracellular domains. It is also known that LAMP-2A is distributed rather ubiquitously, while LAMP-2B is expressed predominantly in striated muscle and brain. Clone H4B4 has been shown to stain for LAMP-2 in skeletal muscle. This information leads us to believe that this antibody will react with LAMP-2B, though we have not verified this through in-house testing.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.