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Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
![Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)](/ps/datasheet/images/ab25631_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP2 antibody [H4B4] - Lysosome Marker(ab25631)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP2 antibody [H4B4] - Lysosome Marker(ab25631)](\ps\datasheet\images\25\ab25631\LAMP2-Primary-antibodies-ab25631-1.jpg)
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Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
![Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)](/ps/datasheet/images/25/ab25631/LAMP2-Primary-antibodies-ab25631-6.jpg)
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Product Name
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LAMP2 antibody [H4B4] - Lysosome Marker
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See all LAMP2 - Lysosomal Marker antibodies (16)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [H4B4] to LAMP2 - Lysosome Marker
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Immunogen
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The details of the immunogen for this antibody are not available.
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Reacts with
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Hu, AGMk
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Does not react with
(species key)
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Ms, Rat
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Specificity
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Human CD107b/LAMP-2
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use 1µg for 10 cells.
ICC/IF: Use at an assay dependent concentration.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 19837699).
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Myeloid Progenitor
Cardiovascular >> Heart >> Autophagy >> Autophagosome
Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
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Relevance
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Lysosomes are membrane bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to post-translational modification, Lysosome Associated Membrane Protein 2 (LAMP2) is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino terminal intra lysosomal domain, a hydrophobic transmembrane domain, and a short carboxyl terminal cytoplasmic tail. Studies suggest that LAMP2 may be involved in leucocyte adhesion.
LAMP2 has recently been shown to be important in autophagy - the process of bulk degradation of cellular proteins. Human LAMP-2 deficiency causes Danon’s disease which is associated with the accumulation of autophagic
material in striated myocytes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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H4B4
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: None
Constituents: 100mM Borate. pH 8
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LAMP2 antibody [H4B4] - Lysosome Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LAMP2 antibody [H4B4] - Lysosome Marker images, references, products related to ab25631 and other tools.
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LAMP2 antibody [H4B4] - Lysosome Marker images:
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 Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
ab25631 at 0.5µg/ml staining human HEK cells by immunocytochemistry. The cells were fixed with paraformaldehyde and incubated with the antibody for 1 hour. Bound antibody was detected using a goat anti-mouse IgG Alexa-Fluor ® 568. In the confocal image ab25631 labelling in red shows a distribution consistent with the location of lysosomes. Blue nuclear counterstain is present. This image is courtesy of an Abreview submitted by Randal Moldrich on 31 March 2006.
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP2 antibody [H4B4] - Lysosome Marker(ab25631)
Ab25631 staining human normal placenta. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
ab25631 staining LAMP2 in human THP-1 cells by Immunocytochemistry/ immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocking with 1% BSA at 220C for 2 hours was done. Samples were incubated with primary antibody (1/400: in PBST and 1% BSA) for 16 hour at 40C. An Alexa Fluor ® 568-conjugated goat polyclonal to mouse IgG, diluted 1/500 was used as secondary antibody. Merge figure shows the brightfield image and flourescent image combined.
This image is a courtesy of Anonimous Abreview.
See Abreview
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Western blot - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
![Western blot - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)](/ps/datasheet/images/25/ab25631/LAMP2-Primary-antibodies-ab25631-7.jpg)
LAMP2 antibody [H4B4] - Lysosome Marker (ab25631) at 1/500 dilution + whole cell lysate prepared from THP-1 macrophages at 30 µg
Secondary
Goat anti-mouse IgG conjugated to HRP at 1/2000 dilution
developed using the ECL technique
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Primary antibody incubated for 12 hours at 4°C.Gel running conditions: 12%Blocked with 5% milk for 1 hour at 25°C. This image was kindly supplied by Daniel Rodriguez by Abreview
See Abreview
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References for LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)
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This product has been used in: (two most recent references)
Colle MA et al. Efficient intracerebral delivery of AAV5 vector encoding human ARSA in non-human primate. Hum Mol Genet 19:147-58 (2010). IHC-FoFr; Macaque Monkey. PubMed: 19837699
Llarena M et al. Different mechanisms of recognition and ER retention by transmembrane transcription factors CREB-H and ATF6. Traffic 11:48-69 (2010). ICC/IF. PubMed: 19883396
See all 3 publication references for this product.
If you publish research using ab25631 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to LAMP2
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LAMP2 antibody [H4B4] - Lysosome Marker - more information
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LAMP2 antibody [H4B4] - Lysosome Marker - related products:
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LAMP2 antibody [H4B4] - Lysosome Marker - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)](/index.html?datasheet=25631#ab25631_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LAMP2 antibody [H4B4] - Lysosome Marker(ab25631)](/index.html?datasheet=25631#LAMP2-Primary-antibodies-ab25631-1.jpg)
![Immunocytochemistry/ Immunofluorescence - LAMP2 antibody [H4B4] - Lysosome Marker (ab25631)](/index.html?datasheet=25631#LAMP2-Primary-antibodies-ab25631-6.jpg)
