Anti-LAMP2 antibody (ab37024)

Western blot - LAMP2 antibody (ab37024)

Lane 1 : Anti-LAMP2 antibody (ab37024) at 1 µg/ml
Lane 2 : Anti-LAMP2 antibody (ab37024) at 2 µg/ml

Lane 1 : HepG2 cell lysate.
Lane 2 : HepG2 cell lysate.

Lysates/proteins at 15 µg per lane.


Predicted band size : 45 kDa


Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.

Immunocytochemistry/ Immunofluorescence - LAMP2 antibody (ab37024)

Immunocytochemistry of LAMP-2 in HepG2 cells with ab37024 at 10 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP2 antibody (ab37024)

ab 37024 staining LAMP2 in human placenta.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry - LAMP2 antibody (ab37024)

ab37024 staining mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 25⁰C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3 ^®-conjugated goat polyclonal to rabbit IgG (H&L) was used at dilution at 1/200 as secondary antibody.

This image is a courtesy of Anonymous Abreview

Immunohistochemistry (Frozen sections) - LAMP2 antibody (ab37024)

IHC-Fr image of human brain section stained with ab37024. The brain sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab37024, 1µg/ml) overnight at +4ºC. The secondary antibody was Alexa Fluor®488 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom