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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Overview
Product name
Anti-LAMP2 antibody
See all LAMP2 products (21) ...
Description
Rabbit polyclonal to LAMP2
Tested applications
WB, ICC, IHC-P, ICC/IF, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
Synthetic peptide (Human) - a 17 amino acid peptide from the C-terminus of human LAMP-2.
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Myeloid Progenitor
Cardiovascular >> Heart >> Autophagy >> Autophagosome
Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 110 kDa (predicted molecular weight: 45 kDa).
ICC: Use at an assay dependent dilution.
IHC-P: Use a concentration of 4 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
IHC-Fr: Use a concentration of 1 µg/ml
Target
Function
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Tissue specificity
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Involvement in disease
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
Sequence similarities
Belongs to the LAMP family.
Post-translational
modifications
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Target information above from: UniProt accessionP13473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD107 antigen-like family member B antibody
- CD107b antibody
- CD107b antigen antibody
see all
Database links
- Entrez Gene: 3920 Human
- Entrez Gene: 16784 Mouse
- Omim: 309060 Human
- SwissProt: P13473 Human
- SwissProt: P17047 Mouse
- Unigene: 496684 Human
- Unigene: 486 Mouse
Anti-LAMP2 antibody images:
Western blot - LAMP2 antibody (ab37024)
Lane 1 : Anti-LAMP2 antibody (ab37024) at 1 µg/ml
Lane 2 : Anti-LAMP2 antibody (ab37024) at 2 µg/ml
Lane 1 : HepG2 cell lysate.
Lane 2 : HepG2 cell lysate.
Lysates/proteins at 15 µg per lane.
Predicted band size : 45 kDa
Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.
Immunocytochemistry/ Immunofluorescence - LAMP2 antibody (ab37024)
Immunocytochemistry of LAMP-2 in HepG2 cells with ab37024 at 10 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAMP2 antibody (ab37024)
ab 37024 staining LAMP2 in human placenta.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry - LAMP2 antibody (ab37024)
ab37024 staining mouse primary astrocytes by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.05% saponin and blocking with 10% serum was performed for 20 minutes at 250C. Samples were incubated with primary antibody (1/150: in 10% goat serum / PBS) for 1 hour at 25°C. An Abcam`s ab6939, Cy3 ®-conjugated goat polyclonal to rabbit IgG (H&L) was used at dilution at 1/200 as secondary antibody.
This image is a courtesy of Anonymous Abreview
Immunohistochemistry (Frozen sections) - LAMP2 antibody (ab37024)
IHC-Fr image of human brain section stained with ab37024. The brain sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton100 for 1h to permeabilise the cells and to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab37024, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®488 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
References for Anti-LAMP2 antibody (ab37024)
This product has been referenced in:
- Novgorodov SAet al. Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ loading capacity and promotes apoptosis. J Biol Chem 286:4644-58 (2011). WB; Rat.Read more (PubMed: 21148554) »
- Faustino JVet al. Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke. J Neurosci 31:12992-3001 (2011).Read more (PubMed: 21900578) »
See all 9 publications for this product
Publishing research using ab37024? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"