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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Overview
Product name
Anti-LAMP2 antibody
See all LAMP2 products (21) ...
Description
Rabbit polyclonal to LAMP2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH-conjugated synthetic peptide selected from the C-terminal region of human LAMP2.
Positive control
Y79 cell line lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Myeloid Progenitor
Cardiovascular >> Heart >> Autophagy >> Autophagosome
Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
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Western blot - LAMP2 antibody (ab71066)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
Tissue specificity
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
Involvement in disease
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
Sequence similarities
Belongs to the LAMP family.
Post-translational
modifications
O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Target information above from: UniProt accessionP13473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD107 antigen-like family member B antibody
- CD107b antibody
- CD107b antigen antibody
see all
Database links
- Entrez Gene: 3920 Human
- Omim: 309060 Human
- SwissProt: P13473 Human
- Unigene: 496684 Human
Anti-LAMP2 antibody images:
Western blot - LAMP2 antibody (ab71066)
Anti-LAMP2 antibody (ab71066) at 1/60 dilution + Y79 cell line lysate at 35 µg/ml
Predicted band size : 45 kDa
Observed band size : 45 kDa
References for Anti-LAMP2 antibody (ab71066)
ab71066 has not yet been referenced specifically in any publications.
Publishing research using ab71066? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"