LAMP2 antibody (ab71066)

Product Name 

LAMP2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to LAMP2

Immunogen 

KLH-conjugated synthetic peptide selected from the C-terminal region of human LAMP2.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
WB: 1/50 - 1/100. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Y79 cell line lysates

Cellular localization 

Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Myeloid Progenitor
Cardiovascular >> Heart >> Autophagy >> Autophagosome
Immunology >> Secreted Molecules >> Other secreted molecules
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome

Relevance 

Lysosomes are membrane bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to post-translational modification, Lysosome Associated Membrane Protein 2 (LAMP2) is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino terminal intra lysosomal domain, a hydrophobic transmembrane domain, and a short carboxyl terminal cytoplasmic tail. Studies suggest that LAMP2 may be involved in leucocyte adhesion.
LAMP2 has recently been shown to be important in autophagy - the process of bulk degradation of cellular proteins. Human LAMP-2 deficiency causes Danon’s disease which is associated with the accumulation of autophagic
material in striated myocytes.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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