Anti-LAMP2 antibody (ab84844)

Overview

• Product nameAnti-LAMP2 antibodySee all LAMP2 primary antibodies ...
• Description
  Rabbit polyclonal to LAMP2
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Cow, Macaque Monkey, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human LAMP2.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab98943.)

• Positive controlThis antibody gave a positive signal in Human lung tissue lysate as well as the following whole cell lysates: HepG2; THP1; HeLa; Jurkat.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy
  • Autophagosome

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Myeloid Progenitor

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Heart
  • Autophagy
  • Autophagosome

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Lysosome

  • Immunology
  • Secreted Molecules
  • Other secreted molecules

Applications

Target

• FunctionImplicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.
• Tissue specificityIsoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver.
• Involvement in diseaseDefects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes.
• Sequence similaritiesBelongs to the LAMP family.
• Post-translational
  modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.
• Cellular localizationCell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Target information above from: UniProt accession P13473 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3920 Human
  • Omim: 309060 Human
  • SwissProt: P13473 Human
  • Unigene: 496684 Human

• Alternative names
  CD107 antigen-like family member B antibodyCD107b antibodyLAMP 2 antibody

  LAMP 2C antibodyLAMP-2 antibodyLAMP2 antibodyLAMP2_HUMAN antibodyLAMPB antibodyLGP110 antibodyLysosome associated membrane protein 2 antibodyLysosome-associated membrane glycoprotein 2 antibodyLysosome-associated membrane protein 2 antibodyMAC3 antibody

  see all

Anti-LAMP2 antibody images

• 

  Western blot - LAMP2 antibody (ab84844)
  All lanes : Anti-LAMP2 antibody (ab84844) at 1/250 dilution
  
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
  Lane 3 : Lung (Human) Tissue Lysate
  Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 110 kDa
  Observed band size : 110 kDa
  Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 8 minutes