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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human LAMP2.
(Peptide available as ab98943.)
Our Abpromise guarantee covers the use of ab84844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human LAMP2 peptide (ab98943).|
ab84844 staining LAMP2 in post-mortem human tissue. Control case : Tissue from cortical regions from a patient who died without neurodegenerative disorder. PDD Case: Tissue from cortical regions from a patient who died with Parkinson's disease dementia. Tissue samples were fixed with Formaldehyde, permeabilized with Triton-X, and antigen retrieval was heat mediated with citric acid. Samples were blocked in 3% BSA for 1 hour at 21ºC, before incubation with ab84844 at 1/400 for 18 hours at 4°C. A goat-anti-rabbit secondary conjugated to HRP was used at 1/1000.
ab84844 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"