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Good afternoon,
I bought an antibody from abcam,
Anti-LAMP3 antibody (ab83659)
I did a western blot using SH-SY5Y whole cell lysate, and I got two bands, one between 37 and 50 kDa and other between 50 and 75 kDa. I see in the insert that the antibody detects two bands, of 44 and 64 kDa.
I read that the predicted molecular weight is of 44 kDa, and I would like to ask what is the other band, the heavy band? |
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ANSWER: |
Thank you for contacting us. LAMP3 is glycosylated and the higher MW band is likely a glycosylated form of the protein.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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