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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Overview
Product name
Anti-LAMP3 antibody
See all LAMP3 products (2) ...
Description
Rabbit polyclonal to LAMP3
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT) of Human LAMP3 (NP_055213).
YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT
Positive control
MCF7 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
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Western blot - LAMP3 antibody (ab83659)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 and 64 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
Tissue specificity
Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
Sequence similarities
Belongs to the LAMP family.
Cellular localization
Lysosome membrane.
Target information above from: UniProt accessionQ9UQV4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD208 antibody
- CD208 antigen antibody
- DC LAMP antibody
see all
Database links
- Entrez Gene: 27074 Human
- Omim: 605883 Human
- SwissProt: Q9UQV4 Human
- Unigene: 518448 Human
Anti-LAMP3 antibody images:
Western blot - LAMP3 antibody (ab83659)
Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-LAMP3 antibody (ab83659)
ab83659 has not yet been referenced specifically in any publications.
Publishing research using ab83659? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"