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Western blot - LAMP3 antibody (ab83659)
(enlarge)
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Product Name
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LAMP3 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to LAMP3
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Immunogen
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Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT) of Human LAMP3 (NP_055213).
BLAST 'YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 and 64 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MCF7 cell lysate.
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Cellular localization
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Lysosome membrane; Single pass type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
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Relevance
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LAMP3 might change the lysosome function after the transfer of peptide MHC class II molecules to the surface of dendritic cells. It's over expression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer. Expressed in lymphoid organs and dendritic cells and lung. Up regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 27074 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 50 µl of distilled water for a final antibody concentration of 1 mg/ml.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LAMP3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LAMP3 antibody images, references, products related to ab83659 and other tools.
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LAMP3 antibody images:
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 Western blot - LAMP3 antibody (ab83659)
LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Search PubMed (MEDLINE) for references to LAMP3
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LAMP3 antibody - more information
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Customer reviews (feedback) regarding LAMP3 antibody |
Customer FAQs regarding LAMP3 antibody |
Protocols for LAMP3 antibody |
Price and availability of products related to LAMP3 antibody |
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LAMP3 antibody - related products:
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LAMP3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about LAMP3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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