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Western blot - LAMP3 antibody (ab83659)
Western blot - LAMP3 antibody (ab83659)
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Product Name 

LAMP3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to LAMP3

Immunogen 

Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT) of Human LAMP3 (NP_055213).
BLAST 'YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 and 64 kDa (predicted molecular weight: 44 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 cell lysate.

Cellular localization 

Lysosome membrane; Single pass type I membrane protein.

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome

Relevance 

LAMP3 might change the lysosome function after the transfer of peptide MHC class II molecules to the surface of dendritic cells. It's over expression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer. Expressed in lymphoid organs and dendritic cells and lung. Up regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.

 

Alternative names LAMP3 antibody (ab83659)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

27074    (Human)

SwissProt

    

Q9UQV4    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Lyophilised
Add 50 µl of distilled water for a final antibody concentration of 1 mg/ml.

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LAMP3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for LAMP3 antibody images, references, products related to ab83659 and other tools.

 

LAMP3 antibody images:

Product image  Western blot - LAMP3 antibody (ab83659)

Western blot - LAMP3 antibody (ab83659)

LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 44 kDa
Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)


Gel concentration 12%

 
 

Search PubMed (MEDLINE) for references to LAMP3

 

LAMP3 antibody - more information

Customer reviews (feedback) regarding LAMP3 antibody

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Price and availability of products related to LAMP3 antibody

 

LAMP3 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for LAMP3 antibody

ab29539

  

MCF7 (Human breast adenocarcinoma cell line) Membrane Lysate - tumor cell line

 

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ab6564

  

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Agarose antibody

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10nm Gold antibody

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Chromeo™ 488 antibody

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LAMP3 antibody - other tools:

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