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Read our guarantee »Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Anti-LAMP3 antibody
See all LAMP3 products (2) ...
Rabbit polyclonal to LAMP3
ELISA, WBmore details
Reacts with
Human
Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT) of Human LAMP3 (NP_055213).
YSQPTAAATV QDIKKPVQQP AKQAPHQTLA ARFMDGHITF QTAATVKIPT
MCF7 cell lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Western blot - LAMP3 antibody (ab83659)
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Our Abpromise guarantee covers the use of ab83659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 44 and 64 kDa (predicted molecular weight: 44 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.
Belongs to the LAMP family.
Lysosome membrane.
Target information above from: UniProt accessionQ9UQV4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - LAMP3 antibody (ab83659)

Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
ab83659 has not yet been referenced specifically in any publications.
Publishing research using ab83659? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

Anti-LAMP3 antibody (ab83659) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 44 kDa
Observed band size : 44,64 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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