Overview

  • Product nameAnti-LAP1 antibody [RL13]
    See all LAP1 primary antibodies
  • Description
    Mouse monoclonal [RL13] to LAP1
  • Tested applicationsIHC-P, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to Rat LAP1. This is a Pore complex-lamina fraction isolated from rat liver nuclear envelopes.

Properties

Applications

Our Abpromise guarantee covers the use of ab2737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50.
ICC/IF 1/100.
IP Use at an assay dependent concentration.
WB 1/200.

Target

Anti-LAP1 antibody [RL13] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on rat breast tissue. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 (1:50) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on rat colon tissue. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 (1:50) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on rat lymph node tissue. Antigen retreived was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2737 (1:50) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of LAP1 using ab2737 shows staining in NS-1 Cells.

References for Anti-LAP1 antibody [RL13] (ab2737)

This product has been referenced in:
  • Liao JJ  et al. Distinctive T cell-suppressive signals from nuclearized type 1 sphingosine 1-phosphate G protein-coupled receptors. J Biol Chem 282:1964-72 (2007). ICC/IF . Read more (PubMed: 17121832) »
  • Yang L  et al. Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase. J Cell Biol 139:1077-87 (1997). Read more (PubMed: 9382857) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"