Anti-LAP2 alpha antibody [3A3] (ab66588)
Key features and details
- Mouse monoclonal [3A3] to LAP2 alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-LAP2 alpha antibody [3A3]
See all LAP2 alpha primary antibodies -
Description
Mouse monoclonal [3A3] to LAP2 alpha -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in HeLa whole cell lysates and post-mitotic HeLa lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3A3 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).
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Notes |
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WB
Use a concentration of 10 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa). |
Target
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Relevance
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent- and salt-resistant nucleoskeletal structure. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 7112 Human
- Omim: 188380 Human
- SwissProt: P42166 Human
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Alternative names
- Isoforms beta/gamma antibody
- Lamina associated polypeptide 2 antibody
- LAP2 antibody
see all
Images
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All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 75 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab66588 has been referenced in 2 publications.
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450
- Qi R et al. The lamin-A/C-LAP2a-BAF1 protein complex regulates mitotic spindle assembly and positioning. J Cell Sci 128:2830-41 (2015). PubMed: 26092935