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Anti-LAP2 antibody [6E10] (ab11823)

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Overview

Product name

Anti-LAP2 antibody [6E10]
See all LAP2 products (6) ...

Description

Mouse monoclonal [6E10] to LAP2

Tested applications

IP, WB, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse, Cow, Xenopus laevis

Immunogen

Synthetic peptide: GEQRKDVYVQLYLQHLTARNRP, corresponding to amino acids 29-50 of Human LAP2, common to all isoforms.

GEQRKDVYVQLYLQHLTARNRP

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Epitope

The epitope recognized by the antibody resides within the N-terminus (amino acids 29-50), common to all human and mouse LAP2/TMPOs

Positive control

Whole extract of cultured human chronic myelogenous leukemia K562 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

6E10

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class V >> Lamins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Use at an assay dependent dilution.
WB: Use at a concentration of 2 - 4 µg/ml. Detects bands of approximately 76 (alpha), 50 (beta, singlet or doublet), 45 (epsilon), 42 (delta), 38 (gamma, singlet or doublet) and 25 kDa (zeta).
IP: Use at a concentration of 15 µg/ml (from PubMed:17213199).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.

Cellular localization

Nuclear

Alternative names

  • Lamina associated polypeptide 2 antibody
  • LAP 2 antibody
  • LEM domain containing 4 antibody
  • LEMD4 antibody
  • thymopoietin antibody
  • Thymopoietin isoform alpha antibody
  • Thymopoietin related peptide isoform alpha antibody
  • Thymopoietin related peptide isoforms beta/gamma antibody
  • Thymopoietin, isoforms beta/gamma antibody
  • TMPO antibody
  • TP alpha antibody
  • TP antibody
  • TP beta/gamma antibody
  • TPRP isoform alpha antibody
  • TPRP isoforms beta/gamma antibody
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Database links

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References for Anti-LAP2 antibody [6E10] (ab11823)

This product has been referenced in:

  • Finlan LE  et al. Recruitment to the nuclear periphery can alter expression of genes in human cells. PLoS Genet 4:e1000039 (2008). WB; Rat.Read more (PubMed: 18369458) »
  • Snyers L & Schöfer C Lamina-associated polypeptide 2alpha forms complexes with heat shock proteins Hsp70 and Hsc70 in vivo. Biochem Biophys Res Commun 368:767-71 (2008). WB; Human.Read more (PubMed: 18261988) »

See all 3 publications for this product

Publishing research using ab11823? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"