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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-LAP3 antibody - Aminoterminal end
See all LAP3 products (4) ...
Description
Rabbit polyclonal to LAP3 - Aminoterminal end
Tested applications
IHC-P, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the N-terminus of human LAP3, conjugated to KLH.
Positive control
HepG2 cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Blood Pressure regulation
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100.Detects a band of approximately 56 , 95 kDa (predicted molecular weight: 56 kDa).
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Relevance
LAP3 is presumably involved in the processing and regular turnover of intracellular proteins. It catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Reduced enzymatic activity of LAP3 may cause hypertension.
Cellular localization
Cytoplasmic
Alternative names
- Cytosol aminopeptidase antibody
- LAP 3 antibody
- LAP antibody
see all
Database links
- Entrez Gene: 51056 Human
- Omim: 170250 Human
- SwissProt: P28838 Human
Anti-LAP3 antibody - Aminoterminal end images:
Western blot - LAP3 antibody - Aminoterminal end (ab71417)
Anti-LAP3 antibody - Aminoterminal end (ab71417) at 1/60 dilution + HepG2 cell lysate at 35 µg
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAP3 antibody - Aminoterminal end (ab71417)
ab71417 at 1/50 dilution staining LAP3 - Aminoterminal end in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-LAP3 antibody - Aminoterminal end (ab71417)
ab71417 has not yet been referenced specifically in any publications.
Publishing research using ab71417? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"