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Products:Cancer >> Tumor biomarkers >> Other
Overview
Product name
Anti-LAPTM4B antibody
Description
Rabbit polyclonal to LAPTM4B
Tested applications
ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (YPNSIQEYIR QLPPNFPYRD DVMSVNPTCL VLIILLFISI ILTFKGYLIS) of human LAPTM4B (NP_060877).
YPNSIQEYIR QLPPNFPYRD DVMSVNPTCL VLIILLFISI ILTFKGYLIS
Positive control
293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 35 kDa.
Target
Relevance
LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has an active role in disease progression of malignant cells and is involved in cell proliferation and multidrug resistance. The genetic polymorphism of LAPTM4B is a potential risk factor for the development of colon cancer and gastric cancer. The research results also indicated that LAPTM4B may be a clinically useful prognostic indicator for ovarian carcinoma and may play a role in human hepatocellular carcinoma.
Cellular localization
Endomembrane system; Multi pass membrane protein.
Alternative names
- LAPTM4beta antibody
- LC27 antibody
- Lysosomal associated protein transmembrane 4 beta antibody
see all
Database links
- Entrez Gene: 55353 Human
- Entrez Gene: 114128 Mouse
- Entrez Gene: 315047 Rat
- SwissProt: Q86VI4 Human
- SwissProt: Q91XQ6 Mouse
- SwissProt: Q5U1W4 Rat
Anti-LAPTM4B antibody images:
Western blot - LAPTM4B antibody (ab82810)
Anti-LAPTM4B antibody (ab82810) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence-LAPTM4B antibody(ab82810)
ICC/IF image of ab82810 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82810, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-LAPTM4B antibody (ab82810)
ab82810 has not yet been referenced specifically in any publications.
Publishing research using ab82810? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"