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Overview

  • Product nameLARP1 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 679-1019 (341 amino acids) of Human LARP1 with an N terminal His tag. Predicted mwt: 40 kDa; UniProt ID = Q6PKG0 (isoform 2).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 84 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesGAPEPSTIARSLPTTVPESPNYRNTRTPRTPRTPQLKDSS QTSRFYPVVKEGRTLDAK MPRKRKTRHSSNPPLESHVG WVMDSREHRPRTASISSSPSEGTPTVGSYGCTPQSLPK FQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQE MNTLFRFWSFFLRDHFNK KMYEEFKQLALEDAKEGYRY GLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQL YGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVD PPMGEEGNHKRHSVVAGG GGGEGRKRCPSQSSSRPAAM ISQPPTPPTGQPVREDAKWTSQHSNTQTLGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92152 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        1810024J12Rik3110040D16RikKIAA0731
        La related proteinLa ribonucleoprotein domain family, member 1LARPMGC19556mKIAA0731rCG_32791RGD1306683RP23-293I3.7
      see all
  • RelevanceLARP1 belongs to the LARP family and contains 1 HTH La-type RNA-binding domain.

    • LARP1 protein (Tagged-His Tag) images

    • SDS-PAGE - LARP1 protein (Tagged-His Tag) (ab92152)
      SDS-PAGE - LARP1 protein (Tagged-His Tag) (ab92152)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for LARP1 protein (Tagged-His Tag) (ab92152)

    ab92152 has not yet been referenced specifically in any publications.

    Publishing research using ab92152 ? Please let us know so that we can cite the reference in this datasheet

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