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Anti-LASP1 antibody (ab1301)

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Overview

Product name

Anti-LASP1 antibody
See all LASP1 products (6) ...

Description

Goat polyclonal to LASP1

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Pig

Immunogen

Synthetic peptide: NPNCARCGKIVYP, corresponding to N terminal amino acids 2-14 of Human LASP1.

NPNCARCGKI VYP

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

General notes

Principal Names: LASP1 (LIM and SH3 protein 1), MLN50. GenBank Accession Number: NP006139 LocusLink ID: 3927 Please note that one of our customers has had problems getting this antibody to detect endogenous Lasp-1 in 2 different human cell lines. Furthermore, a LASP-1/GFP fusion protein overexpressed in a cell line could not be detected with this antibody and yet was detected with our Rabbit Polyclonal to GFP (ab290). For further information please see the enquiries section of the online datasheet. We are currently conducting more QC tests on this product and are interested to hear from other researchers using it. 24 March, 2004.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Primary antibody notes

This protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins

  • Western blot - LASP1 antibody (ab1301)Western blot - LASP1 antibody (ab1301) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Western Blot-Approx 30kDa band seen in Mouse Brain extracts.
Recommended for use at 0.5-2µg/ml.

Target

Function

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.

Sequence similarities

Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.

Cellular localization

Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.

Target information above from: UniProt accessionQ14847 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • LASP 1 antibody
  • LASP-1 antibody
  • LASP1 antibody
  • LASP1_HUMAN antibody
  • LIM and SH3 domain protein 1 antibody
  • LIM and SH3 protein 1 antibody
  • Metastatic lymph node gene 50 protein antibody
  • MLN 50 antibody
  • MLN50 antibody
  • OTTHUMP00000164238 antibody
  • OTTHUMP00000164239 antibody
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Database links

see all

Anti-LASP1 antibody images:

  Western blot - LASP1 antibody (ab1301)

Western blot - LASP1 antibody (ab1301)

LASP1 staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

References for Anti-LASP1 antibody (ab1301)

This product has been referenced in:

  • Li Ket al. Organelle proteomics of rat synaptic proteins: correlation-profiling by isotope-coded affinity tagging in conjunction with liquid chromatography-tandem mass spectrometry to reveal post-synaptic density specific proteins. J Proteome Res 4:725-33 (2005).Read more (PubMed: 15952719) »

See 1 publication for this product

Publishing research using ab1301? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"