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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-LASP1 antibody
See all LASP1 products (6) ...
Description
Goat polyclonal to LASP1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Orangutan
Immunogen
Synthetic peptide: PERRDSQDGSSYR, from the internal region of human LASP1
PERRDSQDGS SYR
Positive control
Human kidney lysates
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Western blot - LASP1 antibody (ab65944)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
Peptide ELISA: antibody detection limit dilution 1:32,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
Target information above from: UniProt accessionQ14847
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
see all
Database links
- Entrez Gene: 3927 Human
- Omim: 602920 Human
- SwissProt: Q14847 Human
- Unigene: 548018 Human
- Unigene: 728766 Human
Anti-LASP1 antibody images:
Western blot - LASP1 antibody (ab65944)
Anti-LASP1 antibody (ab65944) at 0.1 µg/ml + Human kidney lysates at 35 µg
developed using the ECL technique
Predicted band size : 29 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour
References for Anti-LASP1 antibody (ab65944)
ab65944 has not yet been referenced specifically in any publications.
Publishing research using ab65944? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"