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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-LASP1 antibody
See all LASP1 products (6) ...
Description
Mouse monoclonal to LASP1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length human LASP1 protein (AAH12460) with a 26 kDa tag.
Positive control
LASP1 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Western blot - LASP1 antibody (ab76882)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 30 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 2 nebulin repeats.
Contains 1 SH3 domain.
Cellular localization
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Associated with the F-actin rich cortical cytoskeleton.
Target information above from: UniProt accessionQ14847
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LASP 1 antibody
- LASP-1 antibody
- LASP1 antibody
see all
Database links
- Entrez Gene: 3927 Human
- Omim: 602920 Human
- SwissProt: Q14847 Human
- Unigene: 548018 Human
- Unigene: 728766 Human
Anti-LASP1 antibody images:
Western blot - LASP1 antibody (ab76882)
All lanes : Anti-LASP1 antibody (ab76882) at 5 µg/ml
Lane 1 : LASP1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 30 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
References for Anti-LASP1 antibody (ab76882)
ab76882 has not yet been referenced specifically in any publications.
Publishing research using ab76882? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"