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Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-LASS2 antibody
See all LASS2 products (3) ...
Description
Rabbit polyclonal to LASS2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (EKTRLRAPPN ATLEHFYLTS GKQPKQVEVE LLSRQSGLSG RQVERWFRRR) of Human LASS2 (NP_071358).
EKTRLRAPPN ATLEHFYLTS GKQPKQVEVE LLSRQSGLSG RQVERWFRRR
Positive control
Human fetal brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Signal Transduction >> Metabolism >> Lipid metabolism
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Western blot - LASS2 antibody (ab85567)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab85567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Target
Relevance
LASS2 (LAG1 homolog, ceramide synthase 2) has sequence similarity to yeast longevity assurance gene 1. It is thought to be involved in sphingolipid synthesis. Expression of LASS2 is transiently increased during the period of active myelination and is specifically localized to white matter tracts of the brain, including the Schwann cells of sciatic nerves suggesting that LASS2 is important for the synthesis of dihydroceramide used for synthesis of myelin sphingolipids. Expression of LASS2 in hepatocellular carcinoma cell lines suppresses the growth of cancer cells.
Cellular localization
Nucleus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Alternative names
- CerS 2 antibody
- CerS2 antibody
- FLJ10243 antibody
see all
Database links
- Entrez Gene: 29956 Human
- Entrez Gene: 76893 Mouse
- Omim: 606920 Human
- SwissProt: Q96G23 Human
- SwissProt: Q924Z4 Mouse
- Unigene: 713103 Human
Anti-LASS2 antibody images:
Western blot - LASS2 antibody (ab85567)
Anti-LASS2 antibody (ab85567) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 30 kDa,35 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-LASS2 antibody (ab85567)
ab85567 has not yet been referenced specifically in any publications.
Publishing research using ab85567? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"