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Western blot - LASS4 antibody (ab66512)
Western blot - LASS4 antibody (ab66512)
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Product Name 

LASS4 antibody

 

See all LASS4 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to LASS4

Immunogen 

A region within synthetic peptide: PIRRKIKPNP VLEKYFLRMK QCPEETQMVL LASQCGLTLR QTQRWFRRRR, corresponding to N terminal amino acids 73-122 of Human LASS4
BLAST 'PIRRKIKPNP VLEKYFLRMK QCPEETQMVL LASQCGLTLR QTQRWFRRRR ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA titre using peptide based assay: 1/312500.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

NIH3T3 cell lysate.

Cellular localization 

Nuclear membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism

Relevance 

LASS4 is a member of the LASS (longevity assurance homologue) family which are involved in determining life span in yeast, by an unknown molecular mechanism. Members of this family are highly conserved from yeasts to mammals. Five mouse and human LASS family members, namely LASS1, LASS2, LASS4, LASS5 and LASS6, have been identified and characterized.
LASS proteins are thought to be involved in ceramide synthesis. Overproduction of a single LASS protein results in an increase in one or more specific ceramide species, which differ in fatty-acid chain length (LASS4; C22:0 and C24:0). LASS proteins possess specific fatty acyl-CoA preferences, but all can use sphingosine or dihydrosphingosine as the long-chain base substrate. LASS family members exhibit different tissue-specific expression patterns, which may contribute to the production of tissue-specific ceramides.

 

Alternative names LASS4 antibody (ab66512)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

79603    (Human)
67260    (Mouse)
304208    (Rat)

SwissProt

    

Q9HA82    (Human)
Q9D6J1    (Mouse)

Unigene

    

515111    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LASS4 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for LASS4 antibody images, references, products related to ab66512 and other tools.

 

LASS4 antibody images:

Product image  Western blot - LASS4 antibody (ab66512)

Western blot - LASS4 antibody (ab66512)

LASS4 antibody (ab66512) at 2.5 µg/ml + NIH3T3 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 46 kDa
Observed band size : 46 kDa

 
 

Search PubMed (MEDLINE) for references to LASS4

 

LASS4 antibody - more information

Customer reviews (feedback) regarding LASS4 antibody

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Price and availability of products related to LASS4 antibody

 

LASS4 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for LASS4 antibody

ab14874

  

NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear lysate

 

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Texas Red ® antibody

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Agarose antibody

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10nm Gold antibody

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5nm Gold antibody

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15nm Gold antibody

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ab27238

  

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Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

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Chromeo™ 642 antibody

 

Other LASS4 Antibodies

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LASS4 antibody - other tools:

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