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Western blot - LASS4 antibody (ab66512)
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Product Name
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LASS4 antibody
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See all LASS4 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to LASS4
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Immunogen
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A region within synthetic peptide: PIRRKIKPNP VLEKYFLRMK QCPEETQMVL LASQCGLTLR QTQRWFRRRR, corresponding to N terminal amino acids 73-122 of Human LASS4
BLAST 'PIRRKIKPNP VLEKYFLRMK QCPEETQMVL LASQCGLTLR QTQRWFRRRR ' with  or 
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Reacts with
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA titre using peptide based assay: 1/312500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH3T3 cell lysate.
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Cellular localization
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Nuclear membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
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Relevance
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LASS4 is a member of the LASS (longevity assurance homologue) family which are involved in determining life span in yeast, by an unknown molecular mechanism. Members of this family are highly conserved from yeasts to mammals. Five mouse and human LASS family members, namely LASS1, LASS2, LASS4, LASS5 and LASS6, have been identified and characterized.
LASS proteins are thought to be involved in ceramide synthesis. Overproduction of a single LASS protein results in an increase in one or more specific ceramide species, which differ in fatty-acid chain length (LASS4; C22:0 and C24:0). LASS proteins possess specific fatty acyl-CoA preferences, but all can use sphingosine or dihydrosphingosine as the long-chain base substrate. LASS family members exhibit different tissue-specific expression patterns, which may contribute to the production of tissue-specific ceramides.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LASS4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LASS4 antibody images, references, products related to ab66512 and other tools.
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LASS4 antibody images:
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 Western blot - LASS4 antibody (ab66512)
LASS4 antibody (ab66512) at 2.5 µg/ml + NIH3T3 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa
Observed band size : 46 kDa
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Search PubMed (MEDLINE) for references to LASS4
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LASS4 antibody - more information
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Customer reviews (feedback) regarding LASS4 antibody |
Customer FAQs regarding LASS4 antibody |
Protocols for LASS4 antibody |
Price and availability of products related to LASS4 antibody |
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LASS4 antibody - related products:
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LASS4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about LASS4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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