Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-LASS4 antibody
See all LASS4 products (3) ...
Description
Rabbit polyclonal to LASS4
Specificity
ab70121 detects endogenous levels of total LASS4 protein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from an internal sequence of human LASS4.
Positive control
COLO, Jurkat and HUVEC cell extracts and human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab70121 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain.
Cellular localization
Nucleus membrane. Endoplasmic reticulum membrane.
Target information above from: UniProt accessionQ9HA82
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ12089 antibody
- CerS4 antibody
- LAG1 homolog ceramide synthase 4 antibody
see all
Database links
- Entrez Gene: 79603 Human
- SwissProt: Q9HA82 Human
- Unigene: 515111 Human
Anti-LASS4 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LASS4 antibody (ab70121)
ab70121 at 1/50 dilution staining LASS4 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - LASS4 antibody (ab70121)
All lanes : Anti-LASS4 antibody (ab70121) at 1/500 dilution
Lane 1 : COLO cell extracts
Lane 2 : Jurkat cell extracts
Lane 3 : HUVEC cell extracts
Lane 4 : HUVEC cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 46 kDa
Observed band size : 46 kDa
References for Anti-LASS4 antibody (ab70121)
ab70121 has not yet been referenced specifically in any publications.
Publishing research using ab70121? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"