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Products:Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Overview
Product name
Anti-LAT (phospho Y191) antibody
See all LAT products (19) ...
Description
Rabbit polyclonal to LAT (phospho Y191)
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic phospho peptide (Human) derived from a region of LAT that contains tyrosine 191.
Positive control
Stimulated Jurkat E6.1 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated LAT. The final product is generated by affinity chromatography using a LAT-derived peptide that is phosphorylated at tyrosine 191.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
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Western blot - LAT (phospho Y191) antibody (ab4478)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab4478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1.0 µg/ml.Detects a band of approximately 36 -38 kDa.
Target
Function
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Tissue specificity
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
Post-translational
modifications
Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
Cellular localization
Cell membrane. Present in lipid rafts.
Target information above from: UniProt accessionO43561
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- p36 38 antibody
- pp36 antibody
- 36 kDa phospho tyrosine adapter protein antibody
see all
Database links
- Entrez Gene: 27040 Human
- Entrez Gene: 16797 Mouse
- Entrez Gene: 81511 Rat
- Omim: 602354 Human
- SwissProt: O43561 Human
- SwissProt: O54957 Mouse
- SwissProt: Q546H1 Mouse
- SwissProt: Q80UR6 Mouse
see all
Anti-LAT (phospho Y191) antibody images:
Western blot - LAT (phospho Y191) antibody (ab4478)
Peptide Competition: Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL phospho LAT (Tyr 191) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho LAT (Tyr 191) completely blocks the antibody signal, thereby demonstrating the specificity of the antibody, and stimulation inducedtyrosine phosphorylation of phospho LAT (Tyr 191). This antibody does not cross-react with LAT pY132, pY171 or pY226 (data not shown).
References for Anti-LAT (phospho Y191) antibody (ab4478)
This product has been referenced in:
- Haas Aet al. Intracellular Phospho-Flow cytometry reveals novel insights into TCR proximal signaling events. A comparison with Western blot. Cytometry A 73:799-807 (2008). WB; Human.Read more (PubMed: 18548611) »
See 1 publication for this product
Publishing research using ab4478? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"