Overview

  • Product name
    Anti-LAT (phospho Y200) antibody [EP983(2)Y]
    See all LAT primary antibodies
  • Description
    Rabbit monoclonal [EP983(2)Y] to LAT (phospho Y200)
  • Specificity
    The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 200 of human LAT (long isoform).

  • Positive control
    • Jurkat cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
  • Application notes
    ICC: 1/100 - 1/250.
    WB: 1/5000 - 1/10000. Predicted molecular weight: 28 kDa.

    Is unsuitable for Flow Cyt, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
    • Tissue specificity
      Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
    • Post-translational
      modifications
      Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ.
      Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
    • Cellular localization
      Cell membrane. Present in lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • 36 kDa phospho tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adapter protein antibody
      • 36 kDa phospho-tyrosine adaptor protein antibody
      • LAT 1 antibody
      • lat antibody
      • LAT_HUMAN antibody
      • LAT1 antibody
      • Linker for activation of T cells antibody
      • Linker for activation of T cells family member 1 antibody
      • linker for activation of T cells, transmembrane adaptor antibody
      • Linker for activation of T-cells family member 1 antibody
      • p36 38 antibody
      • p36-38 antibody
      • pp36 antibody
      see all

    Images

    • All lanes : Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : ~35 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Huang SC  et al. Lipid raft assembly and Lck recruitment in TRAIL costimulation mediates NF-?B activation and T cell proliferation. J Immunol 186:931-9 (2011). Read more (PubMed: 21160038) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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