Anti-LATS1/WARTS antibody (ab70561)
Key features and details
- Rabbit polyclonal to LATS1/WARTS
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LATS1/WARTS antibody
See all LATS1/WARTS primary antibodies -
Description
Rabbit polyclonal to LATS1/WARTS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Dog, Chimpanzee, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human LATS1/WARTS aa 50-100.
Database link: O95835 -
Positive control
- WB: Whole cell lysate from HeLa Cells, mouse CT26.WT and TCMK-1 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70561 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/5000 - 1/15000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/5000 - 1/15000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. -
Tissue specificity
Expressed in all adult tissues examined except for lung and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Post-translational
modificationsAutophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy. -
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 476241 Dog
- Entrez Gene: 9113 Human
- Entrez Gene: 16798 Mouse
- Omim: 603473 Human
- SwissProt: O95835 Human
- SwissProt: Q8BYR2 Mouse
- Unigene: 549084 Human
- Unigene: 34083 Mouse
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Alternative names
- h-warts antibody
- Large tumor suppressor homolog 1 antibody
- LATS large tumor suppressor homolog 1 antibody
see all
Images
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All lanes : Anti-LATS1/WARTS antibody (ab70561) at 0.2 µg/ml
Lane 1 : CT26.WT whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 127 kDa
Exposure time: 3 minutes -
All lanes : Anti-LATS1/WARTS antibody (ab70561) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Predicted band size: 127 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 200 kDa, 268 kDa, 71 kDa. We are unsure as to the identity of these extra bands. -
Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70561 at 3 µg/mg of lysate (lane 1).
LATS1/WARTS was also immunoprecipitated with an other LATS1/WARTS antibody (lanes 2) and with ab70562 (lane 3) using 3 µg/mg lysate.
Lane 4: Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1/WARTS was performed using ab70561 at 0.1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab70561 has been referenced in 20 publications.
- Chen T et al. α-Hederin Inhibits the Proliferation of Hepatocellular Carcinoma Cells via Hippo-Yes-Associated Protein Signaling Pathway. Front Oncol 12:839603 (2022). PubMed: 35311132
- Wang L et al. Toxoplasma gondii causes changes in the host's expression of cancer-associated miRNAs. Oncol Lett 23:149 (2022). PubMed: 35350589
- Wang Y et al. Transcriptional Repression of Ferritin Light Chain Increases Ferroptosis Sensitivity in Lung Adenocarcinoma. Front Cell Dev Biol 9:719187 (2021). PubMed: 34765600
- Wang W et al. Parthenolide plays a protective role in the liver of mice with metabolic dysfunction-associated fatty liver disease through the activation of the HIPPO pathway. Mol Med Rep 24:N/A (2021). PubMed: 33955510
- Du T et al. Regulatory effect of microRNA-223-3p on breast cancer cell processes via the Hippo/Yap signaling pathway. Oncol Lett 22:516 (2021). PubMed: 33986876