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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab70565? Please let us know so that we can cite the reference in this datasheet
ab70565 has been referenced in 1 publications.
- Murakami H et al. LATS2 is a tumor suppressor gene of malignant mesothelioma. Cancer Res 71:873-83 (2011). IHC-P; Human. PubMed: 21245096
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - LATS2 antibody (ab70565)
All lanes : Anti-LATS2 antibody (ab70565) at 0.4 µg/ml
Lane 1 : HeLa cell whole lysate at 50 µg
Lane 2 : HeLa cell whole lysate at 15 µg
Lane 3 : HeLa cell whole lysate at 5 µg
Predicted band size : 120 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Immunoprecipitation - LATS2 antibody (ab70565)
Detection of human LATS2 by Immunoprecipitation in HeLa whole cell lysate (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70565 at 3 µg/mg of lysate.
Subsequent blotting of immunoprecipitate for the detection of LATS2 was performed using 1µg/ml of ab70565. Detection was by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LATS2 antibody(ab70565)
ab70565 (2µg/ml) staining LATS2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in pneumocytes.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
LATS2 antibody for WB in Human (70565)
LATS2 antibody for Western blot in Human (70565)
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