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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-LATS2 antibody
See all LATS2 products (4) ...
Description
Rabbit polyclonal to LATS2
Tested applications
IHC-P, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human LATS2 (NP_055387.1)
Positive control
Whole cell lysate from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000 - 1/5000.Detects a band of approximately 150 kDa (predicted molecular weight: 120 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
Tissue specificity
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Post-translational
modifications
Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.
Target information above from: UniProt accessionQ9NRM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ13161 antibody
- Kinase phosphorylated during mitosis antibody
- Kinase phosphorylated during mitosis protein antibody
see all
Database links
- Entrez Gene: 26524 Human
- Omim: 604861 Human
- SwissProt: Q9NRM7 Human
- Unigene: 78960 Human
Anti-LATS2 antibody images:
Western blot - LATS2 antibody (ab70565)
All lanes : Anti-LATS2 antibody (ab70565) at 0.4 µg/ml
Lane 1 : HeLa cell whole lysate at 50 µg
Lane 2 : HeLa cell whole lysate at 15 µg
Lane 3 : HeLa cell whole lysate at 5 µg
Predicted band size : 120 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Immunoprecipitation - LATS2 antibody (ab70565)
Detection of human LATS2 by Immunoprecipitation in HeLa whole cell lysate (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70565 at 3 µg/mg of lysate.
Subsequent blotting of immunoprecipitate for the detection of LATS2 was performed using 1µg/ml of ab70565. Detection was by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-LATS2 antibody(ab70565)
ab70565 (2µg/ml) staining LATS2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in pneumocytes.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-LATS2 antibody (ab70565)
This product has been referenced in:
- Murakami Het al. LATS2 is a tumor suppressor gene of malignant mesothelioma. Cancer Res 71:873-83 (2011). IHC-P; Human.Read more (PubMed: 21245096) »
See 1 publication for this product
Publishing research using ab70565? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"