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Western blot - LATS2 antibody (ab70565)
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Immunoprecipitation - LATS2 antibody (ab70565)
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Product Name
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LATS2 antibody
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See all LATS2 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to LATS2
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Immunogen
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Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human LATS2 (NP_055387.1)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2-5µg/mg of lysate.
WB: 1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 120 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from HeLa cells.
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Cellular localization
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At the centrosomes during interphase, early prophase and cytokinesis, the spindle poles during mitosis, and to the midbody during cytokinesis.
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
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Relevance
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LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LATS2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LATS2 antibody images, references, products related to ab70565 and other tools.
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LATS2 antibody images:
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 Western blot - LATS2 antibody (ab70565)
All lanes : LATS2 antibody (ab70565) at 0.4 µg/ml
Lane 1 : HeLa cell whole lysate at 50 µg
Lane 2 : HeLa cell whole lysate at 15 µg
Lane 3 : HeLa cell whole lysate at 5 µg
Predicted band size : 120 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
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Immunoprecipitation - LATS2 antibody (ab70565)
Detection of human LATS2 by Immunoprecipitation in HeLa whole cell lysate (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70565 at 3 µg/mg of lysate.
Subsequent blotting of immunoprecipitate for the detection of LATS2 was performed using 1µg/ml of ab70565. Detection was by chemiluminescence.
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Search PubMed (MEDLINE) for references to LATS2
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LATS2 antibody - more information
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Customer reviews (feedback) regarding LATS2 antibody |
Customer FAQs regarding LATS2 antibody |
Protocols for LATS2 antibody |
Price and availability of products related to LATS2 antibody |
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LATS2 antibody - related products:
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LATS2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about LATS2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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