![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody [mAbcam54073] (ab54073) image](#LATS2-Primary-antibodies-ab54073-1.jpg)
![Immunocytochemistry/ Immunofluorescence - Anti-LATS2 antibody [mAbcam54073] (ab54073) image](#LATS2-Primary-antibodies-ab54073-2.jpg)
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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-LATS2 antibody [mAbcam54073]
See all LATS2 products (4) ...
Description
Mouse monoclonal [mAbcam54073] to LATS2
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LATS2.
(Peptide available as ab59815.)
Positive control
This antibody gave a positive signal in Hela Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mAbcam54073
Myeloma
Sp2/0-Ag14
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 10 µg/mlDetects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).(Milk block is recommended.)
IHC-P: Use a concentration of 5 µg/ml
Target
Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
Tissue specificity
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Post-translational
modifications
Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.
Target information above from: UniProt accessionQ9NRM7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ13161 antibody
- Kinase phosphorylated during mitosis antibody
- Kinase phosphorylated during mitosis protein antibody
see all
Database links
- Entrez Gene: 26524 Human
- Entrez Gene: 50523 Mouse
- Entrez Gene: 305922 Rat
- Omim: 604861 Human
- SwissProt: Q9NRM7 Human
- SwissProt: Q7TSJ6 Mouse
- Unigene: 78960 Human
- Unigene: 347899 Mouse
Anti-LATS2 antibody [mAbcam54073] images:
Western blot - LATS2 antibody (ab54073)
Anti-LATS2 antibody [mAbcam54073] (ab54073) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 120 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody [mAbcam54073] (ab54073)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody [mAbcam54073] (ab54073)](/ps/datasheet/images/54/ab54073/LATS2-Primary-antibodies-ab54073-1.jpg)
IHC image of ab54073 staining LATS2 in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54073, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-LATS2 antibody [mAbcam54073] (ab54073)
![Immunocytochemistry/ Immunofluorescence - Anti-LATS2 antibody [mAbcam54073] (ab54073)](/ps/datasheet/images/54/ab54073/LATS2-Primary-antibodies-ab54073-2.jpg)
ICC/IF image of ab54073 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54073, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-LATS2 antibody [mAbcam54073] (ab54073)
This product has been referenced in:
- Liu Xet al. MicroRNA-31 Regulated by the Extracellular Regulated Kinase Is Involved in Vascular Smooth Muscle Cell Growth via Large Tumor Suppressor Homolog 2. J Biol Chem 286:42371-80 (2011). WB; Rat.Read more (PubMed: 22020941) »
See 1 publication for this product
Publishing research using ab54073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"