Overview
- Product nameAnti-LC3A/B antibodySee all LC3A/B primary antibodies ...
- DescriptionRabbit polyclonal to LC3A/B
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LC3A/B.
(Peptide available as ab99367.)
- Positive controlThis antibody gave a positive signal in SHSY-5Y and SK N BE Whole Cell Lysates, and in mouse and rat brain tissue lysates.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab84936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa).Can be blocked with LC3A/B peptide (ab99367). |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
Target
- RelevanceA major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.
- Cellular localizationCytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes.
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 81631 Human
- Entrez Gene: 67443 Mouse
- Entrez Gene: 66734 Mouse
- Entrez Gene: 64862 Rat
- Entrez Gene: 362245 Rat
- Entrez Gene: 406466 Zebrafish
- Entrez Gene: 322425 Zebrafish
- Omim: 601242 Human
- Omim: 609604 Human
- SwissProt: Q9GZQ8 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q9CQV6 Mouse
- SwissProt: Q62625 Rat
- SwissProt: Q6XVN8 Rat
- Unigene: 196239 Mouse
see all
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Alternative names
- ATG8F antibodyAutophagy related protein LC3 A antibodyAutophagy related protein LC3 B antibody
- Autophagy related ubiquitin like modifier LC3 A antibodyAutophagy related ubiquitin like modifier LC3 B antibodyLC3 antibodyMAP1 light chain 3 like protein 1 antibodyMAP1 light chain 3 like protein 2 antibodyMAP1A/1B light chain 3 A antibodyMAP1A/1B light chain 3 B antibodyMAP1A/1BLC3 antibodyMAP1A/MAP1B LC3 A antibodyMAP1A/MAP1B LC3 B antibodyMAP1ALC3 antibodyMAP1BLC3 antibodyMAP1LC3A antibodyMAP1LC3B antibodyMicrotubule associated protein 1 light chain 3 alpha antibodyMicrotubule associated protein 1 light chain 3 beta antibodyMicrotubule associated proteins 1A/1B light chain 3 antibodyMicrotubule associated proteins 1A/1B light chain 3A antibodyMicrotubule associated proteins 1A/1B light chain 3B antibody
see all
Anti-LC3A/B antibody images
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Western blot - LC3A/B antibody (ab84936)All lanes : Anti-LC3A/B antibody (ab84936) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45-70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-LC3A/B antibody (ab84936)ICC/IF image of ab84936 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84936, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-LC3A/B antibody (ab84936)All lanes : Anti-LC3A/B antibody (ab84936) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 15 kDa
Additional bands at : 18 kDa,30 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
References for Anti-LC3A/B antibody (ab84936)
ab84936 has not yet been referenced specifically in any publications.
