Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LC3B antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreatic cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
Pancreatic cancer xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS
Dilution
1/250

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti-Rb IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Username

Abcam user community

Verified customer

Submitted Dec 18 2015

Sign up