Immunocytochemistry/ Immunofluorescence abreview for Anti-LC3B antibody

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey Serum in 0.1% PBST
Dilution
1/50

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Red--LC3B; Blue--Nucleus (DAPI)
Username

Dr. Armen Petrosyan

Verified customer

Submitted Jun 03 2016

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