Western blot abreview for Anti-LC3B antibody

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat Hepatocytes)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
20 µg
Specification
Rat Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
45 second(s)
Bands
Specific: Between 17 and 11 kDa Non-specific: 28, 55 and 70 kDa
Username

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Submitted Jun 03 2016

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