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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Overview
Product name
Anti-LC3B antibody
See all LC3B products (8) ...
Description
Rabbit polyclonal to LC3B
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Macaque Monkey, Zebra finch
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LC3B.
(Peptide available as ab87823.)
Positive control
This antibody gave a positive signal in Human, Mouse and Rat Brain Tissue Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Relevance
LC3B was originally identified as microtubule associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule binding domains and the heavy chain independent regulation of LC3B expression might modify MAP1 microtubule binding activity during development. However, LC3B is now thought to also be involved in autophagy.
Cellular localization
Cytoplasmic
Alternative names
- Autophagy related protein LC3 B antibody
- Autophagy related ubiquitin like modifier LC3 B antibody
- MAP1 light chain 3 like protein 2 antibody
see all
Database links
- Entrez Gene: 427559 Chicken
- Entrez Gene: 81631 Human
- Entrez Gene: 67443 Mouse
- Entrez Gene: 64862 Rat
- Omim: 609604 Human
- SwissProt: O41515 Cow
- SwissProt: Q9GZQ8 Human
- SwissProt: Q9CQV6 Mouse
see all
Anti-LC3B antibody images:
Western blot - LC3B antibody (ab63817)
Anti-LC3B antibody (ab63817) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 15 kDa
Additional bands at : 32 kDa,54 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Immunocytochemistry/ Immunofluorescence - LC3B antibody (ab63817)
ICC/IF image of ab63817 stained PC12 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63817, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LC3B antibody (ab63817)
IHC image of LC3B staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63817, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-LC3B antibody (ab63817)
All lanes : Anti-LC3B antibody (ab63817) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 15 kDa
Additional bands at : 30 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
References for Anti-LC3B antibody (ab63817)
ab63817 has not yet been referenced specifically in any publications.
Publishing research using ab63817? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"