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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-LCMT2 antibody
See all LCMT2 products (2) ...
Description
Rabbit polyclonal to LCMT2
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Dog
Immunogen
Synthetic peptide: VEPVLSDWHF LHVGTMAWVR IPVEGEVPEA RHSHSACTWQ GGALIAGGLG , corresponding to a region within C terminal amino acids 505-554 of Human LCMT2 (NP_055608)
VEPVLSDWHF LHVGTMAWVR IPVEGEVPEA RHSHSACTWQ GGALIAGGLG
Positive control
Hela cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Signal Transduction >> Metabolism >> Amino Acids
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Western blot - LCMT2 antibody (ab80937)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 76 kDa.
Target
Relevance
LCMT2 belongs to the methyltransferase superfamily. It is a probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. LCMT2 may methylate the carboxyl group of leucine residues to form alpha-leucine ester residues.
Alternative names
- KIAA0547 antibody
- LCMT 2 antibody
- Leucine carboxyl methyltransferase 2 antibody
see all
Database links
- Entrez Gene: 9836 Human
- Omim: 611246 Human
- SwissProt: O60294 Human
Anti-LCMT2 antibody images:
Western blot - LCMT2 antibody (ab80937)
Anti-LCMT2 antibody (ab80937) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 76 kDa
Observed band size : 76 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-LCMT2 antibody (ab80937)
ab80937 has not yet been referenced specifically in any publications.
Publishing research using ab80937? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"