Anti-LDB1 antibody (ab48573)

Overview

• Product nameAnti-LDB1 antibodySee all LDB1 primary antibodies ...
• Description
  Rabbit polyclonal to LDB1
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Dog, Pig, Zebrafish
• Immunogen

  A region within synthetic peptide: EPTRQQPSKR RKRKMSGGST MSSGGGNTNN SNSKKKSPAS TFALSSQVPD, corresponding to amino acids 251-300 of Human LDB1

  Run BLAST with Run BLAST with
• Positive controlJurkat cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • LIM

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionBinds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
• Tissue specificityExpressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
• Sequence similaritiesBelongs to the LDB family.
• DomainThe dimerization domain is located in the N-terminus.
• Post-translational
  modificationsUbiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q86U70 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8861 Human
  • Entrez Gene: 8861 Human
  • Entrez Gene: 16825 Mouse
  • Entrez Gene: 30576 Zebrafish
  • GenBank: NM_003893 Human
  • GenBank: 26002 Human
  • GenBank: NM_010697 Mouse
  • GenBank: NM_131313 Zebrafish

  • Omim: 603451 Human
  • SwissProt: Q86U70 Human
  • SwissProt: Q86U70 Human
  • SwissProt: P70662 Mouse
  • SwissProt: O73712 Zebrafish
  • SwissProt: Q2YDQ6 Zebrafish
  • Unigene: 454418 Human
  • Unigene: 327442 Mouse
  • Unigene: 598 Zebrafish

  see all

• Alternative names
  Carboxyl Terminal LIM Domain Binding 2 antibodyCarboxyl Terminal LIM Domain Binding 2 antibodyCarboxyl-terminal LIM domain-binding protein 2 antibody

  CLIM 2 antibodyCLIM 2 antibodyCLIM-2 antibodyhLdb1 antibodyLDB-1 antibodyldb1 antibodyLDB1_HUMAN antibodyLIM Domain Binding 1 antibodyLIM Domain Binding 1 antibodyLIM domain binding factor CLIM2 antibodyLIM domain-binding factor CLIM2 antibodyLIM domain-binding protein 1 antibodyNLI antibodyNLI antibodyNuclear LIM Domain Interactor antibodyNuclear LIM Domain Interactor antibodyNuclear LIM interactor antibodyxldb1 antibody

  see all

Anti-LDB1 antibody images

• 

  Western blot - LDB1 antibody (ab48573)
  Anti-LDB1 antibody (ab48573) at 1 µg/ml + Jurkat cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 42 kDa
  Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.