Overview

  • Product nameAnti-LDB1 antibody
    See all LDB1 primary antibodies
  • Description
    Rabbit polyclonal to LDB1
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: EPTRQQPSKR RKRKMSGGST MSSGGGNTNN SNSKKKSPAS TFALSSQVPD, corresponding to amino acids 251-300 of Human LDB1

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab48573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionBinds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.
  • Tissue specificityExpressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the LDB family.
  • DomainThe dimerization domain is located in the N-terminus.
  • Post-translational
    modifications
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carboxyl Terminal LIM Domain Binding 2 antibody
    • Carboxyl Terminal LIM Domain Binding 2 antibody
    • Carboxyl-terminal LIM domain-binding protein 2 antibody
    • CLIM 2 antibody
    • CLIM 2 antibody
    • CLIM-2 antibody
    • hLdb1 antibody
    • LDB-1 antibody
    • ldb1 antibody
    • LDB1_HUMAN antibody
    • LIM Domain Binding 1 antibody
    • LIM Domain Binding 1 antibody
    • LIM domain binding factor CLIM2 antibody
    • LIM domain-binding factor CLIM2 antibody
    • LIM domain-binding protein 1 antibody
    • NLI antibody
    • NLI antibody
    • Nuclear LIM Domain Interactor antibody
    • Nuclear LIM Domain Interactor antibody
    • Nuclear LIM interactor antibody
    • xldb1 antibody
    see all

Anti-LDB1 antibody images

  • Anti-LDB1 antibody (ab48573) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-LDB1 antibody (ab48573)

ab48573 has not yet been referenced specifically in any publications.

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