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Anti-LDB3 antibody (ab40840) at 0.01 µg/ml + Human Muscle lysate (35µg protein in RIPA buffer).
Predicted band size : 77 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Muscle lysates after 0.01µg/ml antibody staining. These bands correspond to earlier findings in literature (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364).
ab40840 at 3.8 µg/ml staining LBD3 in Human Skeletal muscle by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate pH 6, AP-staining.
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