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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-LDB3 antibody
See all LDB3 products (4) ...
Description
Goat polyclonal to LDB3
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Pig
Immunogen
Synthetic peptide: KSQNKPEDEADE, corresponding to Internal sequence amino acids 251-262 of Human LDB3
KSQNKPEDEA DE
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 3 - 6 µg/ml.
WB: Use a concentration of 0.01 - 0.03 µg/ml.Predicted molecular weight: 77 kDa.
Target
Function
May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
Tissue specificity
Expressed primarily in skeletal muscle and to a lesser extent in heart. Also detected in brain and placenta.
Involvement in disease
Defects in LDB3 are the cause of cardiomyopathy dilated type 1C (CMD1C) [MIM:601493]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in LDB3 are the cause of left ventricular non-compaction type 3 (LVNC3) [MIM:601493]. Left ventricular non-compaction is characterized by numerous prominent trabeculations and deep intertrabecular recesses in hypertrophied and hypokinetic segments of the left ventricle.
Defects in LDB3 are the cause of myopathy myofibrillar ZASP-related (MFM-ZASP) [MIM:609452]. A neuromuscular disorder characterized by distal and proximal muscle weakness with signs of cardiomyopathy and neuropathy.
Sequence similarities
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Cellular localization
Cytoplasm > perinuclear region. Cell projection > pseudopodium. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
Target information above from: UniProt accessionO75112
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CMD1C antibody
- CYPHER antibody
- HGNC:15710 antibody
see all
Database links
- Entrez Gene: 479269 Dog
- Entrez Gene: 11155 Human
- Entrez Gene: 24131 Mouse
- Entrez Gene: 100151883 Pig
- Omim: 605906 Human
- SwissProt: E2QW94 Dog
- SwissProt: E2QX48 Dog
- SwissProt: O75112 Human
see all
Anti-LDB3 antibody images:
Western blot - LDB3 antibody (ab40840)
Anti-LDB3 antibody (ab40840) at 0.01 µg/ml + Human Muscle lysate (35µg protein in RIPA buffer).
Predicted band size : 77 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Muscle lysates after 0.01µg/ml antibody staining. These bands correspond to earlier findings in literature (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB3 antibody (ab40840)
ab40840 at 3.8 µg/ml staining LBD3 in Human Skeletal muscle by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate pH 6, AP-staining.
References for Anti-LDB3 antibody (ab40840)
ab40840 has not yet been referenced specifically in any publications.
Publishing research using ab40840? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"