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Western blot - LDB3 antibody (ab40840)
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Product Name
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LDB3 antibody
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See all LDB3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to LDB3
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Immunogen
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Synthetic peptide: KSQNKPEDEADE, corresponding to Internal sequence amino acids 251-262 of Human LDB3
BLAST 'KSQNKPEDEADE' with  or 
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Reacts with
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Hu
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Predicted to react
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Ms, Rat, Dog
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 77 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Localized to the cytoplasm around nuclei and pseudopodia of undifferentiated cells and detected throughout the myotubes of differentiated cells. Colocalizes with ACTN2 at the Z-lines.
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Research areas
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Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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LDB3 (LIM domain-binding protein 3) may function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton. It interactes with various PKC isoforms and with the C-terminal region of ACTN2 via its LIM and PDZ domains respectively. It also interacts with MYOZ1, MYOZ2 and MYOZ3. 6 isoforms are produced by alternative splicing. Defects in LDB3 are a cause of dilated cardiomyopathy 1A, a disorder characterized by cardiac dilation and reduced systolic function, as well as ZASP-related myofibrillar myopathy, an autosomal dominant myopathy that is characterized by distal muscle weakness with signs of cardiomyopathy and neuropathy.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LDB3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LDB3 antibody images, references, products related to ab40840 and other tools.
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LDB3 antibody images:
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 Western blot - LDB3 antibody (ab40840)
LDB3 antibody (ab40840) at 0.01 µg/ml + Human Muscle lysate (35µg protein in RIPA buffer).
Predicted band size : 77 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Muscle lysates after 0.01µg/ml antibody staining. These bands correspond to earlier findings in literature (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364).
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Search PubMed (MEDLINE) for references to LDB3
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LDB3 antibody - more information
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Customer reviews (feedback) regarding LDB3 antibody |
Customer FAQs regarding LDB3 antibody |
Protocols for LDB3 antibody |
Price and availability of products related to LDB3 antibody |
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LDB3 antibody - related products:
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LDB3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about LDB3 antibody |
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