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LDH-Cytotoxicity Assay Kit (400 assays) (ab65391)

Q&A (9)

Overview

  • Product nameLDH-Cytotoxicity Assay Kit (400 assays)See all Lactate dehydrogenase kits ...
  • Tests
    400 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    1h 00m
  • Product overview

    Abcam's LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5-1 hr.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceLactate dehydrogenase (LDH) is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
  • Cellular localizationCytoplasmic
    • Alternative names
        L lactate dehydrogenase A chainL lactate dehydrogenase B chainLactate dehydrogenase A
        Lactate dehydrogenase BLDHALDHBRenal carcinoma antigen NY REN 46Renal carcinoma antigen NY REN 59
      see all

    Applications

    Our Abpromise guarantee covers the use of ab65391 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    LDH-Cytotoxicity Assay Kit (400 assays) images

    • Functional Studies - Lactate Dehydrogenase-Cytotoxicity Assay Kit (ab65391)
      Functional Studies - Lactate Dehydrogenase-Cytotoxicity Assay Kit (ab65391)
      Jurkat cells were cultured in a 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium according to the kit instructions. Light bar: Low control; Dark bar: High control.

    Protocols

    References for LDH-Cytotoxicity Assay Kit (400 assays) (ab65391)

    ab65391 has not yet been referenced specifically in any publications.

    Publishing research using ab65391 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 9 of 9 results for Abreviews and Q&A

    10
    Votes: Highest First

    what is the difference between both kits?
    can one them to be used with tissue culture supernatant only (no cells)?

    Thank you for contacting us.

    I have heard back from the lab with the following information:

    There are numerous differences between these two assay kits. For details, I have attached the datasheets, but here are the main differences:
    ...

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    I have discussed the situation with my PI and we would like a refund. We did a control experiment with the remainder of the kit and we found the same problem. How do we go about getting the refund?
    Thank you,

    Thank you for your reply, and I'm sorry that we could not solve this problem for you.

    I'm happy to issue the refund. Do you have your order number, PO number, or date of the purchase?

    Please let me know if you have any questions or if ...

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    I spoke to you on the phone today (4/3/12) just before 2pm. We are having problems with an LDH assay and you and I were discussing how to analyze the data. I have attached a spreadsheet with the data and additional information. Please email or call me if ...

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    Thank you for your call this week and for your patience while I have been in touch with the lab.

    The scientists at the lab have confirmed that the absorbance value should increase with increasing cytotoxicity, so the data is contradicting what w...

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    Dear technical team,

    Our customer would like to test LDHassay with tissue sample.
    http://www.abcam.com/LDH-Cytotoxicity-Assay-Kit-400-assays-ab65391-support.html

    Is it possible with ab65391?

    Let me know.

    <...

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    Thank you for contacting us and your interest in our products.

    The LDH-Cytotoxicity Assay Kit (ab65391) is designed to measure the levels of LDH in the cell culture supernatants and determines the cytotoxicitybased on this. The kit can be theore...

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    Does this LDH-Cytotoxicity Assay Kit suitable for all species？Including rat and mouse cells.

    Thank you for contacting us. This kit is compatible with mammalian samples and hence will work with mouse and rat samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  ...

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    Dear technical team,   Our customer would like to purchase LDH-Cytotoxicity Assay Kit and I have searched two products on the website.   But I don't know well what the differrences between them, ab65391 and ab65393.   Let me know, please.   Thank you in a...

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    Thank you for your inquiry. There are a few differences between those two kits: With the LDH-Cytotoxicity Assay Kit ab65391 LDH activity is determined by a coupled enzymatic reaction. This assay kit is sensitive, convenient, and precise, and is app...

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    Which of these 2 kits would be suitable for a use on HeLa cells as well as mucus?  

    Merci de nous avoir contactés. Mes collaborateurs m'ont informé que le kit ab102526 est le plus adapté à vos besoins. Le lien vers la fiche technique est : www.abcam.com/ab102526. J'espère que cette information vous est utile. N'hésitez pas à nous...

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    I'm pretty interested in buying your LDH-Cytotoxicity Assay Kit (400 assays) (ab65391). But before placing the order, I want to make sure if we can read the products. We have a plate reader, which is BioTek Synergy 2. Is it good for reading the samples?

    As long as your plate reader has the ability to measure the absorbance of all samples at 490-500 nm, then you will be able to use the plate reader with the LDH-Cytotoxicity assay kit. I believe that the plate reader that you have, does have the ability t...

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    What is the difference between the LDH cytotoxicity assay kits ab65393 and ab65391?

    Thank you for your phone call today and for your questions about our LDH assay kits. The kits do utilize different reactions in order to determine DLH activity, but there are also some other differences. I have summarized the advantages of each kit bel...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"