LDH-Cytotoxicity Assay Kit (400 assays) (ab65391)
- Product nameLDH-Cytotoxicity Assay Kit (400 assays)See all Lactate dehydrogenase kits ...
- Tests400 x 1 assay
- Sample typeAdherent cells, Suspension cells
- Assay typeEnzyme activity
- Assay time1h 00m
- Product overview
Abcam's LDH-Cytotoxicity Assay Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. The LDH-cytotoxicity assay is sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies. Assay takes ~0.5-1 hr.
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Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 400 tests Catalyst (Lyophilized) 1 vial Dye Solution 1 x 45ml
- L lactate dehydrogenase A chainL lactate dehydrogenase B chainLactate dehydrogenase A
- Lactate dehydrogenase BLDHALDHBRenal carcinoma antigen NY REN 46Renal carcinoma antigen NY REN 59
Our Abpromise guarantee covers the use of ab65391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
LDH-Cytotoxicity Assay Kit (400 assays) images
Jurkat cells were cultured in a 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium according to the kit instructions. Light bar: Low control; Dark bar: High control.
References for LDH-Cytotoxicity Assay Kit (400 assays) (ab65391)
ab65391 has not yet been referenced specifically in any publications.