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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-LDHA antibody
See all LDHA products (6) ...
Description
Rabbit polyclonal to LDHA
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human, Zebrafish
Predicted to work with
Mouse, Rat, Rabbit, Chimpanzee, Chinese Hamster
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Lactate Dehydrogenase.
(Peptide available as ab47009.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00338
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 19 protein antibody
- LDH muscle subunit; M LDH antibody
- PIG19 antibody
see all
Database links
- Entrez Gene: 3939 Human
- Entrez Gene: 16828 Mouse
- Entrez Gene: 24533 Rat
- Entrez Gene: 30496 Zebrafish
- Omim: 150000 Human
- SwissProt: P00338 Human
- SwissProt: P06151 Mouse
- SwissProt: P04642 Rat
see all
Anti-LDHA antibody images:
Western blot - LDHA antibody (ab47010)
All lanes : LDHA antibody (ab47010) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - LDHA antibody (ab47010)
ICC/IF image of ab47010 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab47010, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LDHA antibody (ab47010)
IHC image of Lactate Dehydrogenase staining in human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47010, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LDHA antibody (ab47010)
ab47010 at a 1/400 dilution staining LDHA in human skeletal muscle sections by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections) incubated for 20 minutes at 25°C. Heat mediated antigen retrieval used, EDTA ph 9.0, 100°C for 20 minutes. Secondary used undiluted, goat anti-mouse/rabbit IgG conjugated to HRP polymer.
This image is from an anonymous abreview.
References for Anti-LDHA antibody (ab47010)
ab47010 has not yet been referenced specifically in any publications.
Publishing research using ab47010? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"